The Phospho-MEK1-T292 Antibody (CABP1221) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications. By binding specifically to the phosphorylated form of MEK1 at threonine 292, this antibody enables the detection and analysis of MEK1 phosphorylation in various cell types.MEK1 is a critical kinase in the MAPK signaling pathway, playing a key role in cell proliferation, differentiation, and survival. Phosphorylation of MEK1 at threonine 292 is known to regulate its activity and downstream signaling, making it a crucial event in the regulation of cell growth and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-MEK1-T292 Antibody
SKU:
CABP1221
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PRTP G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cytoplasm, Membrane, Nucleus, Peripheral Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method
Affinity purification
Gene ID
5604
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit pAb (CABP1221) at 1:1000 dilution. HeLa cells were treated with Nocodazole (100 ng/ml) at 37℃ for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
