Description
Caveolin-1 (Phospho-Tyr14)Colorimetric Cell-Based ELISA Kit
The Caveolin-1 Phospho-Tyr14 Colorimetric Cell-Based ELISA Kit is a cutting-edge tool for the sensitive and accurate detection of Caveolin-1 phosphorylated at tyrosine 14 in cell lysates. This kit offers high specificity and precision, providing researchers with reliable and reproducible results for a variety of experimental applications.Caveolin-1 is a key player in signal transduction pathways and membrane trafficking, with phosphorylation at tyrosine 14 regulating its function in various cellular processes. Dysregulation of Caveolin-1 has been implicated in several diseases, including cancer, diabetes, and cardiovascular disorders, underscoring its importance as a potential therapeutic target and diagnostic marker.
With its innovative technology and superior performance, the Caveolin-1 Phospho-Tyr14 Colorimetric Cell-Based ELISA Kit is a valuable asset for scientists working in cell biology, signal transduction, and disease research. Trust in this kit for precise measurements and insightful data analysis in your experiments.
| Product Name: | Caveolin-1 (Phospho-Tyr14)Colorimetric Cell-Based ELISA Kit |
| Product Code: | CBCAB00251 |
| ELISA Type: | Cell-Based |
| Target: | Caveolin-1 (Phospho-Tyr14) |
| Reactivity: | Human, Mouse, Rat |
| Dynamic Range: | > 5000 Cells |
| Detection Method: | Colorimetric 450 nm |
| Format: | 2 x 96-Well Microplates |
The Caveolin-1 (Phospho-Tyr14) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Caveolin-1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Caveolin-1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caveolin-1 phosphorylation.
Qualitative determination of Caveolin-1 (Phospho-Tyr14) concentration is achieved by an indirect ELISA format. In essence, Caveolin-1 (Phospho-Tyr14) is captured by Caveolin-1 (Phospho-Tyr14)-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:
| 1. | A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. |
| 2. | Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. |