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Cell Viability, Cytotoxicity & Proliferation Assays

Cell Viability, Cytotoxicity & Proliferation Assay Kits | Assay Genie
Assay Genie · Cell health

Measure cell viability, cytotoxicity & growth

Whether you're screening drugs or testing toxicity, our assays quantify cell health three ways — metabolic viability, membrane-damage cytotoxicity and proliferation — with familiar, reliable microplate protocols.

MTT, CCK-8/WST-8 & LDHMicroplate-reader readyScreening-friendly
7
Assay Kits
3
Read-out Types
MTT
CCK-8 & LDH
HTS
Compatible

Which cell health assay do you need?

Viability, cytotoxicity and proliferation answer different questions. Pick what you want to measure and we'll show the matching kits.

What do you want to measure?
Cell Viability
Metabolic viability (MTT, general)
Cytotoxicity
LDH release & cytotoxicity
Proliferation
CCK-8/WST-8 & MTT proliferation

Cell viability & proliferation range

Filter by read-out or search by assay or product code.

Why choose these assays?

Trusted chemistries for cell-health quantitation.

Three questions, one range

Metabolic viability, membrane-integrity cytotoxicity and proliferation covered.

Familiar & reliable

Classic MTT and modern CCK-8/WST-8 and LDH chemistries you can trust.

Screening-ready

Microplate formats suited to drug screening and toxicity testing.

Simple workflows

Add-and-read designs with straightforward absorbance or fluorescence detection.

Viability, cytotoxicity or proliferation?

These assays answer related but distinct questions. Viability assays (e.g. MTT) report the fraction of metabolically active cells; cytotoxicity assays (e.g. LDH release) report membrane damage and cell death; proliferation assays (e.g. CCK-8/WST-8) report the rate of cell division.

Choosing the right read-out — or combining them — gives an accurate picture of how cells respond to a treatment.

  • Viability: MTT and general viability assays
  • Cytotoxicity: LDH release and cytotoxicity kits
  • Proliferation: CCK-8/WST-8 and MTT proliferation
  • Live/dead discrimination by fluorescence

FAQs

What's the difference between viability, cytotoxicity and proliferation assays?

Viability assays measure metabolically active cells, cytotoxicity assays measure cell death or membrane damage (e.g. LDH release), and proliferation assays measure the rate of cell division. They are often used together.

MTT or CCK-8/WST-8 — which should I use?

Both measure metabolic activity via tetrazolium reduction. CCK-8/WST-8 uses a water-soluble formazan (no solubilisation step) and is generally more convenient and sensitive, while MTT is a long-established, economical standard.

How does an LDH assay measure cytotoxicity?

Lactate dehydrogenase released from cells with damaged membranes is measured in the medium; more LDH indicates more cell death, making it a marker of cytotoxicity.

Are these assays suitable for high-throughput screening?

Yes. The microplate formats and add-and-read workflows suit drug screening and toxicity testing in 96-well and higher-density plates.

Setting up a screening assay?

Our technical team can help you pick the right viability, cytotoxicity or proliferation read-out for your cells.

Global shipping to 80+ countriesPhD-level technical supportValidated, reproducible assaysCited in peer-reviewed research