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Anti-CHAF1B Antibody (CAB14074)

SKU:
CAB14074
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
€129 - €359
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Description

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Antibody Name:Anti-CHAF1B Antibody
Antibody SKU:CAB14074
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SRKN LKRP IAHL PCPG KATL AVRC CPVY FELR PVVE TGVE LMSL PYRL VFAV ASED SVLL YDTQ QSFP FGYV SNIH YHTL SDIS WSSD GAFL AISS TDGY CSFV TFEK DELG IPLK EKPV LNMR TPDT AKKT KSQT HRGS SPGP RPVE GTPA SRTQ DPSS PGTT PPQA RQAP APTV IRDP PSIT PAVK SPLP GPSE EKTL QPSS QNTK AHPS RRVT LNTL QAWS KTTP RRIN LTPL KTDT PPSS VPTS VIST PSTE EIQS ETPG DAQG SPPE LKRP RLDE NKGG TESL DP
Gene ID:8208
Uniprot:Q13112
Cellular Location:Cytoplasm, Nucleus
Calculated MW:61kDa
Observed MW:Refer to figures
Synonyms:CHAF1B, CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Background:Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
UniProt Protein Function:Function: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. Ref.6
UniProt Protein Details:

Subunit structure: Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected.

Subcellular location: Nucleus. Cytoplasm. Note: DNA replication foci. Cytoplasmic in M phase. Ref.7

Developmental stage: Active complex is found in G1, S and G2 phases.

Post-translational modification: Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. Ref.5 Ref.7

Sequence similarities: Belongs to the WD repeat HIR1 family.Contains 7 WD repeats.

NCBI Summary:Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
UniProt Code:Q13112
NCBI GenInfo Identifier:3121829
NCBI Gene ID:8208
NCBI Accession:Q13112.1
UniProt Secondary Accession:Q13112,Q99548,
UniProt Related Accession:Q13112
Molecular Weight:61,493 Da
NCBI Full Name:Chromatin assembly factor 1 subunit B
NCBI Synonym Full Names:chromatin assembly factor 1, subunit B (p60)
NCBI Official Symbol:CHAF1B  
NCBI Official Synonym Symbols:CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7  
NCBI Protein Information:chromatin assembly factor 1 subunit B; CAF-I p60; CAF-1 subunit B; CAF-I 60 kDa subunit; M-phase phosphoprotein 7; chromatin assembly factor I p60 subunit; human chromatin assembly factor-I p60 subunit
UniProt Protein Name:Chromatin assembly factor 1 subunit B
UniProt Synonym Protein Names:Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7
UniProt Gene Name:CHAF1B  
UniProt Entry Name:CAF1B_HUMAN

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