Enhanced Cell Counting Kit 8 (WST-8 / CCK8) (MAES0207)
- Product type:
- Cellular Assay
- Detection Method:
|Product Name:||Enhanced Cell Counting Kit 8 (WST-8 / CCK8)|
|Product Size:||50 Assays, 500 Assays, 10,000 Assays|
|Storage:||4°C for 1 year, -20°C for 2 years|
The Assay Genie Enhanced Cell Counting Kit 8 (WST-8 / CCK8) is a rapid, highly sensitive, non-radioactive colorimetric test kit based on WST-8 and widely used in the detection of cell proliferation and cytotoxicity. WST-8 is an analog of MTT, an upgraded replacement product of MTT. Compared with MTT, WST-8 has a number of advantages. Firstly, the formazan product produced after the reaction is water-soluble and does not require a specific solvent to dissolve it. Secondly, WST-8 is more stable, has a wider linear range, and has higher sensitivity.
Since WST-8 & WST-8 formazan have no cytotoxicity in the cell culture media, additional experiments may be carried out using the same cells from the previous assay. Just wash the cells and continue.
WST-8 is a compound similar to MTT, which can be reduced to orange formazan by some dehydrogenase in mitochondria in the presence of electron coupling reagent. The amount of formazan produced is directly proportional to the number of living cells. By measuring the absorbance at 450 nm, the amount of living cells can be calculated indirectly.
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
|1.||Add 100 µL of cell suspension per well to the 96 well microplate. (Set 2 wells as blank wells, do not seed cells but add 100 µL of culture medium). Incubate the cells at 37°C, 5% CO2 incubator for 24 h.|
|2.||Add 10 µL of different concentrations of drugs to each well according to the experimental design. (For the wells with cells added in step 1, set 2 wells as control wells. Do not add drugs but add 10 µL of culture medium).|
|3.||Incubate the cells for an appropriate time according to your experimental design at 37°C, 5% CO2 incubator.|
|4.||Add 10 µL of CCK-8 Buffer and incubate the cells at 37°C, 5%CO2 incubator for 1˜4 h.|
|5.||Measure the absorbance with microplate reader at 450 nm.|
|6.||Calculate the Cell Survival Rate and Inhibition Rate following the formula in the technical manual.|