The FITC-conjugated Goat anti-Mouse IgG (H+L) (CABS001) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, conjugated with FITC for easy detection, targets mouse IgG, enabling specific and sensitive detection in various experimental applications.The FITC-Conjugated Goat Anti-Mouse IgG (H+L) antibody is suitable for use in techniques such as immunofluorescence, flow cytometry, and immunohistochemistry. Its high reactivity with mouse IgG ensures reliable and reproducible results, making it an essential reagent for studies involving mouse antibodies and immunoglobulins.
This antibody is validated for use in IF/ICC, FC applications and has demonstrated reactivity against Mouse samples.
Product Name:
FITC-conjugated Goat anti-Mouse IgG (H+L)
SKU:
CABS001
Size:
100μL
Reactivity:
Mouse
Conjugate:
FITC. Ex:491nm. Em:516nm.
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
IF/ICCFC
Recommended Dilution:
IF/ICC
1:100 - 1:500
FC
1:50 - 1:200
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2769475
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by a further incubation with FITC Goat Anti-Mouse IgG (H+L) (CABS001, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Mouse isotype control (blue) and non-staining control (Red).The secondary antibody used was FITC Goat Anti-Mouse IgG (H+L) (CABS001) at 1:100.
