Description
Glycogen Synthase Colorimetric Cell-Based ELISA Kit
The Glycogen Synthase Colorimetric Cell-Based ELISA Kit is specially designed for the quantitative detection of glycogen synthase levels in cell lysates and tissue homogenates. This kit offers high sensitivity and specificity, providing accurate and reproducible results for your research needs.Glycogen synthase is a key enzyme involved in glycogen synthesis, playing a crucial role in regulating glucose metabolism and energy storage. Dysregulation of glycogen synthase activity has been linked to various metabolic disorders such as diabetes and obesity, making it an important target for therapeutic interventions.
With the Glycogen Synthase Colorimetric Cell-Based ELISA Kit, researchers can study the expression and activity of glycogen synthase in different cellular and tissue samples, leading to a better understanding of its role in metabolic pathways and disease development. Get reliable and precise data with this easy-to-use kit, perfect for a wide range of experimental applications.
| Product Name: | Glycogen Synthase Colorimetric Cell-Based ELISA Kit |
| Product Code: | CBCAB00678 |
| ELISA Type: | Cell-Based |
| Target: | Glycogen Synthase |
| Reactivity: | Human, Mouse, Rat |
| Dynamic Range: | > 5000 Cells |
| Detection Method: | Colorimetric 450 nmStorage/Stability:4°C/6 Months |
| Format: | 96-Well Microplate |
The Glycogen Synthase Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Glycogen Synthase protein expression profile in cells. The kit can be used for measuring the relative amounts of Glycogen Synthase in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on Glycogen Synthase.
Qualitative determination of Glycogen Synthase concentration is achieved by an indirect ELISA format. In essence, Glycogen Synthase is captured by Glycogen Synthase-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:
| 1. | A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. |
| 2. | Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. |
| Database Information: | Gene ID: 2997, UniProt ID: P13807, OMIM: 138570/611556, Unigene: Hs.386225 |
| Gene Symbol: | GYS1 |
| Sub Type: | None |
| UniProt |