The HCN1 Antibody (CAB16198) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the HCN1 protein, this antibody enables accurate detection and analysis in various cell types, making it well-suited for studies in neuroscience and neurological disorders.HCN1 is a member of the hyperpolarization-activated cyclic nucleotide-gated (HCN) channel family, crucial for regulating neuronal firing rates and pacemaking activity in the brain.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HCN1 Antibody
SKU:
CAB16198
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes.
Purification Method
Affinity purification
Gene ID
348980
RRID
AB_2763650
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HCN1 Rabbit pAb (CAB16198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of NIH/3T3 cells using HCN1 Rabbit pAb (CAB16198) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
