HSP25/HSP27 Antibody [8A7] is a mouse monoclonal antibody directed against HSP25/HSP27, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP, FCM and FACS. Reported reactivity: Human, Mouse, Rat, Bovine, Dog, Guinea Pig (Cavia porcellus), Hamster and Sheep. Supplied as IgG1 Kappa, purified by Protein G.
HSP25 (mouse) and its human homolog HSP27 are members of the small heat shock protein (sHSP) family, characterized by a conserved α-crystallin domain and a variable N-terminal region essential for oligomerization. These proteins form dynamic oligomers ranging from dimers to large multimers (8–40 monomers), with chaperone activity closely tied to their oligomeric state—larger assemblies exhibit potent anti-aggregation functions, while dimers are inactive. HSP27 is predominantly cytoplasmic under basal conditions but rapidly translocates to the nucleus in response to cellular stress, where it may stabilize nuclear structures and DNA. It is also rapidly phosphorylated in response to various stimuli, linking it to second messenger signaling pathways. Functionally, HSP27 acts as an ATP-independent molecular chaperone, preventing protein aggregation and stabilizing partially unfolded proteins, often in coordination with the HSP70 complex. In the nervous system, HSP27 plays a critical role in protecting neurons from proteotoxic stress, apoptosis, and oxidative damage—key features of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. It inhibits apoptotic signaling by blocking the cytochrome c/Apaf-1/procaspase-9 complex and may also influence cytoskeletal dynamics through interactions with actin and myosin. Upregulation of HSP27 correlates with increased phosphorylation and oligomer formation, suggesting a role in stress adaptation, cell differentiation, and potentially growth arrest. These properties make HSP27 a compelling target for therapeutic strategies aimed at enhancing neuronal resilience in neurodegenerative disease. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
WB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~25kDa or ~27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Guarantee:
12 months from date of dispatch
Immunogen:
Human HSP27 peptide
Immunogen Species:
Human
Accession Number:
NP_001532.1
Gene ID:
3315
Swiss-Prot:
P04792
Cellular Localization:
Cytoplasm | Nucleus | Cytoskeleton | Spindle
Alternative Names:
HSPB1, HSP27, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, MKBP, DMPK-binding protein
References:
1. Welch W.J. (1985) J Biol. Chem. 260: 3058-3062. 2. Kim K.K., Kim R., and Kim S. (1998) Nature 394(6693): 595-599. 3. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 4. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 5. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70. 6. Welsh M.J., Wu W., Parvinem M., and Gilmont R.R. (1996) Biol. Of Reprod. 55: 141-151. 7. Sarto C. Binnz P.A. and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 8. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6. 9. Jia, Y. et al. (2001) J. Biol. Chem. 276(43):39911-39918.
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