Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit
The Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit is specifically designed for the accurate detection of ACE2 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results, making it an invaluable tool for various research applications.ACE2 is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance.
It is also known for its interaction with the SARS-CoV-2 virus, making it a critical target for studying COVID-19 and other related diseases. The Human ACE2 ELISA Kit provides researchers with the means to measure ACE2 levels accurately, aiding in the investigation of ACE2-related pathways and potential therapeutic interventions.
Product Name:
Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit
SKU:
HUES01516
Target:
Human ACE2 (Angiotensin I Converting Enzyme 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACE2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACE2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACE2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACE2. You can calculate the concentration of Human ACE2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
90-105
90-103
Average (%)
94
97
95
1:4
Range (%)
88-102
82-93
83-97
Average (%)
93
87
89
1:8
Range (%)
86-98
83-97
85-99
Average (%)
92
89
90
1:16
Range (%)
91-106
78-92
83-96
Average (%)
97
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.25
3.33
11.54
1.32
3.29
11.99
Standard deviation
0.07
0.2
0.54
0.07
0.14
0.65
C V (%)
5.6
6.01
4.68
5.3
4.26
5.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ACE2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACE2 in samples. No significant cross-reactivity or interference between Human ACE2 and analogues was observed.
