Human ACT alpha1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit
The Human ACT (Alpha-1-Actin, Alpha 1 skeletal muscle) CLIA Kit is specifically designed for the quantitative measurement of Alpha-1-Actin levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing accurate and consistent results for various research applications.Alpha-1-Actin is a key protein found in skeletal muscle, playing a crucial role in muscle contraction and function. Abnormal levels of Alpha-1-Actin have been associated with muscle diseases and disorders, making it an important biomarker for studying these conditions and developing potential treatments.
Overall, the Human ACT (Alpha-1-Actin, Alpha 1 skeletal muscle) CLIA Kit provides researchers with a reliable tool for investigating the role of Alpha-1-Actin in skeletal muscle health and disease, ultimately advancing our understanding and treatment of relevant conditions.
Product Name:
Human ACT alpha1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit
SKU:
HUES00194
Target:
Human ACT alpha1 (Actin Alpha 1, Skeletal Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTα1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTα1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTα1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACTα1. You can calculate the concentration of Human ACTα1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
100-114
95-111
Average (%)
97
108
102
1:4
Range (%)
86-101
87-102
100-117
Average (%)
92
93
108
1:8
Range (%)
92-109
97-109
86-98
Average (%)
100
102
93
1:16
Range (%)
89-104
94-107
96-109
Average (%)
96
101
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
106
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
217.16
354.1
1555.53
218.09
327.99
1586.24
Standard deviation
24.1
37.71
98.31
27.92
32.73
153.23
C V (%)
11.1
10.65
6.32
12.8
9.98
9.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACTα1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACTα1 in samples. No significant cross-reactivity or interference between Human ACTα1 and analogues was observed.
