Human ALDOA (Aldolase A, Fructose Bisphosphate) CLIA Kit
The Human ALDOA (Aldolase A Fructose Bisphosphate) CLIA Kit is specifically designed for the quantitative detection of ALDOA levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and reproducible results, making it an ideal tool for a variety of research applications.ALDOA is a critical enzyme involved in glycolysis, playing a key role in energy production and metabolism. Dysregulation of ALDOA has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.
As such, ALDOA serves as an important biomarker for studying these diseases and exploring potential therapeutic interventions.With the Human ALDOA CLIA Kit, researchers can precisely measure ALDOA levels in human samples, providing valuable insights into disease pathology and potential treatment strategies. This kit offers reliability and performance, enabling researchers to advance their understanding of ALDOA-related pathways and diseases.
Product Name:
Human ALDOA (Aldolase A, Fructose Bisphosphate) CLIA Kit
SKU:
HUES00224
Target:
Human ALDOA (Aldolase A, Fructose Bisphosphate)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ALDOA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALDOA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALDOA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ALDOA. You can calculate the concentration of Human ALDOA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
92-109
91-105
Average (%)
95
100
97
1:4
Range (%)
102-118
103-116
84-99
Average (%)
109
109
91
1:8
Range (%)
92-108
85-97
87-102
Average (%)
98
91
94
1:16
Range (%)
90-101
94-106
94-105
Average (%)
95
99
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.21
146.44
357.42
53.66
159.56
364.82
Standard deviation
6.12
12.65
38.85
6.26
17.52
32.1
C V (%)
11.5
8.64
10.87
11.67
10.98
8.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ALDOA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ALDOA in samples. No significant cross-reactivity or interference between Human ALDOA and analogues was observed.
