Human BCL2L1/BCL-X (Bcl-2 Like Protein 1) ELISA Kit
The Human BCL2L1 (BCL-x) ELISA Kit is a powerful tool for the quantitative detection of BCL2L1 (BCL-x) levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.BCL2L1 (BCL-x) is a key protein that regulates apoptosis, or programmed cell death, and is important for maintaining cell survival. Dysregulation of BCL2L1 has been implicated in a variety of diseases, including cancer and autoimmune disorders.
As such, measuring BCL2L1 levels can provide valuable insights into disease mechanisms and potential therapeutic interventions.With its user-friendly design and robust performance, the Human BCL2L1 (BCL-x) ELISA Kit is a valuable asset for researchers studying apoptosis, cancer biology, and immunology. Unlock the potential of your research with this reliable and versatile assay.
Product Name:
Human BCL2L1/BCL-X (Bcl-2 Like Protein 1) ELISA Kit
SKU:
HUES01736
Target:
Human BCL2L1/BCL-X (Bcl-2 Like Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BCL2L1/BCL-X. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BCL2L1/BCL-X and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BCL2L1/BCL-X, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BCL2L1/BCL-X. You can calculate the concentration of Human BCL2L1/BCL-X in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
94-109
100-113
Average (%)
102
99
106
1:4
Range (%)
89-103
83-97
81-96
Average (%)
94
89
88
1:8
Range (%)
92-105
84-96
82-93
Average (%)
100
90
88
1:16
Range (%)
89-103
83-96
85-99
Average (%)
95
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.02
8.95
1.14
1.97
8.29
Standard deviation
0.06
0.11
0.27
0.06
0.08
0.36
C V (%)
5.66
5.45
3.02
5.26
4.06
4.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BCL2L1/BCL-X concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BCL2L1/BCL-X in samples. No significant cross-reactivity or interference between Human BCL2L1/BCL-X and analogues was observed.
