Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
The Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit is a reliable and accurate tool for the measurement of BMP-2 levels in human samples including serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, ensuring consistent and precise results for various research applications.BMP-2 is a key protein involved in bone and cartilage formation, playing a crucial role in skeletal development and regeneration. Dysregulation of BMP-2 has been implicated in various musculoskeletal disorders, making it a valuable biomarker for understanding these conditions and developing therapeutic interventions.With the Human BMP-2 ELISA Kit, researchers can confidently study the role of BMP-2 in bone health, osteoporosis, and other related diseases, leading to advancements in the field of regenerative medicine and personalized treatment strategies.
Product Name:
Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
SKU:
HUES01298
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
Serum BMP-2 and osteocalcin levels, and CT Hounsfield unit post hyperbaric oxygen therapy in patients with cleft lip and palate post alveolar bone graft: A case study