Human BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit
The Human BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit is specifically designed for the accurate measurement of BMP-7 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Bone Morphogenetic Protein 7, also known as osteogenic protein-1, is a key factor in bone and cartilage development, playing a crucial role in regulating cell differentiation and tissue regeneration. BMP-7 has also been implicated in various disease processes, including bone disorders, kidney diseases, and cancer, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions. With its advanced technology and dependable performance, the Human BMP-7 ELISA Kit from Assay Genie is an essential tool for researchers studying the role of BMP-7 in health and disease, providing valuable insights into its biological functions and potential clinical applications.
Product Name:
Human BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit
SKU:
HUES01300
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.