The Human C3 Complement Component 3 CLIA Kit is specifically designed for the precise measurement of C3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.C3 is a key protein in the complement system, playing a critical role in immune response and inflammation. Dysregulation of C3 levels is associated with various diseases such as autoimmune disorders, infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential treatment options.
With the Human C3 Complement Component 3 CLIA Kit, researchers can delve deeper into the role of C3 in disease pathogenesis and progression, paving the way for novel therapeutic interventions and personalized medicine approaches. Order your kit today and unlock new insights into the complex interplay of the immune system and disease states.
Product Name:
Human C3 (Complement Component 3) CLIA Kit
SKU:
HUES00501
Target:
Human C3 (Complement Component 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C3. You can calculate the concentration of Human C3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
101-112
103-115
Average (%)
96
106
109
1:4
Range (%)
99-110
95-110
99-115
Average (%)
105
101
106
1:8
Range (%)
90-101
93-104
94-109
Average (%)
96
99
99
1:16
Range (%)
95-110
94-108
85-96
Average (%)
102
101
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-110
100
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
90-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.12
13.59
38.98
5.24
12.56
38.79
Standard deviation
0.45
1.37
3.27
0.43
0.97
3.13
C V (%)
8.79
10.08
8.39
8.21
7.72
8.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C3 in samples. No significant cross-reactivity or interference between Human C3 and analogues was observed.
