The Human C5a Complement C5a ELISA Kit is specially designed for the precise measurement of C5a complement levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring trustworthy and reproducible results for a variety of research purposes. C5a complement is a vital component of the complement system that plays a key role in inflammation and immune response. Elevated levels of C5a have been associated with various inflammatory conditions, including autoimmune diseases, sepsis, and allergic reactions.
By accurately measuring C5a levels, researchers can gain valuable insights into the mechanisms underlying these conditions and develop potential therapeutic interventions. Overall, the Human C5a Complement C5a ELISA Kit is a valuable tool for researchers studying inflammation, immunity, and related diseases, providing reliable data to advance our understanding of these complex biological processes.
Product Name:
Human C5a/Complement C5a ELISA Kit
SKU:
HUFI00086
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-94%
90-104%
94-103%
EDTA Plasma (n = 5)
85-100%
88-100%
86-99%
Heparin Plasma (n = 5)
80-87%
86-97%
88-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
89-105
96
EDTA Plasma (n = 5)
91-99
95
Heparin Plasma (n = 5)
92-105
100
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set s
