Human betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit
The Human betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit is designed for the quantitative detection of Human betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) levels in various biological samples. Beta-Thromboglobulin (β-TG/CXCL7/PBP/NAP-2) is a chemokine of the CXC family stored in platelet alpha-granules and released upon platelet activation. Elevated plasma β-TG concentrations serve as a sensitive marker of in vivo platelet activation and thrombotic activity, reflecting heightened thrombus formation risk. It is widely used to study platelet hyperactivity in cardiovascular diseases, diabetes mellitus, atherosclerosis, and to evaluate antiplatelet therapy efficacy in clinical research. Assay Genie's Human betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 18.75 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including plasma. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Human betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit
SKU:
AEES00031
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
98-113
97-115
95-107
Average (%)
104
105
102
1:4
Range (%)
94-109
100-113
92-109
Average (%)
102
106
99
1:8
Range (%)
98-112
96-110
96-112
Average (%)
106
102
104
1:16
Range (%)
95-109
101-116
97-109
Average (%)
102
107
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
89-105
96
Cell Culture Media (n=8)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.83
310.74
956.63
87.92
299.83
928.22
Standard deviation
4.24
15.35
51.47
5.7
21.11
76.58
C V (%)
4.38
4.94
5.38
6.48
7.04
8.25
Uniport ID:
P02775
Sample type & Sample volume:
Plasma; 100 μL
Specificity:
This kit recognizes Human βTG/PBP/CXCL7/NAP2 in samples.No significant cross-reactivity or interference between Human βTG/PBP/CXCL7/NAP2 and analogues was observed
