The Human Eomesodermin (Eomes) ELISA Kit offered by Assay Genie is a valuable tool for researchers studying the role of Eomesodermin in various biological processes. Eomesodermin is a transcription factor that plays a key role in the development of T cells, natural killer cells, and other immune cells. This ELISA kit allows for the accurate measurement of Eomesodermin levels in human samples, providing researchers with important insights into its expression and function in different physiological and pathological conditions.
With its high sensitivity and specificity, this kit guarantees precise and reliable results, making it a valuable asset for any laboratory conducting research on Eomesodermin and its implications in health and disease. Whether you are studying immune responses, cell differentiation, or cancer progression, the Human Eomesodermin ELISA Kit from Assay Genie is an essential tool for advancing your research and discovering new therapeutic targets. Order yours today and unlock the potential of Eomesodermin in your studies.
Product Name:
Human Eomesodermin (EOMES) ELISA Kit
Product Code:
HUDL00952
Size:
96 Assays
Target:
EOMES
Synonyms:
TBR2, T-Box Brain 2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
