The Human Fibrinogen Beta CLIA Kit is a reliable and accurate tool for measuring fibrinogen beta levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results for various research applications.Fibrinogen beta is an important protein that plays a key role in the blood clotting process and is associated with various diseases and disorders such as cardiovascular diseases and inflammation.
By accurately measuring fibrinogen beta levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially develop new therapeutic interventions.Overall, the Human Fibrinogen Beta CLIA Kit is a valuable tool for researchers studying the role of fibrinogen beta in health and disease, providing accurate and reliable data for further research and clinical applications.
Product Name:
Human FG beta (Fibrinogen Beta) CLIA Kit
SKU:
HUES01127
Target:
Human FG beta (Fibrinogen Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGβ. You can calculate the concentration of Human FGβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
97-114
96-114
Average (%)
108
104
104
1:4
Range (%)
87-103
88-104
91-107
Average (%)
94
95
98
1:8
Range (%)
93-106
84-96
87-102
Average (%)
98
90
95
1:16
Range (%)
98-111
87-101
95-109
Average (%)
105
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.57
7.05
0.87
2.55
6.99
Standard deviation
0.09
0.19
0.5
0.1
0.24
0.78
C V (%)
9.78
7.39
7.09
11.49
9.41
11.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGβ in samples. No significant cross-reactivity or interference between Human FGβ and analogues was observed.
