Human Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) ELISA Kit
The Human Growth Arrest and DNA Damage Inducible Protein Alpha (GADD45A) ELISA Kit is a powerful tool for the quantitative measurement of GADD45A levels in human biological samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.GADD45A is a key protein involved in cellular stress responses, DNA repair, and cell cycle regulation. Its dysregulation has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, the GADD45A ELISA Kit is essential for studying the role of this protein in disease pathogenesis and for developing potential diagnostic and therapeutic strategies.Overall, the GADD45A ELISA Kit provides researchers with a valuable tool for investigating the molecular mechanisms underlying disease development and progression, offering insights that may lead to the development of novel treatment options.
Product Name:
Human Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) ELISA Kit
Product Code:
HUDL01137
Size:
96 Assays
Target:
GADD45a
Synonyms:
DDIT1, GADD45, DNA damage-inducible transcript 1 protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
