Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit
The Human Intercellular Adhesion Molecule 1 (ICAM-1/CD54) ELISA Kit is a cutting-edge assay designed for the precise quantitative detection of ICAM-1 levels in various human biological samples. ICAM-1, a cell surface glycoprotein, plays a crucial role in mediating adhesion processes and immune responses, particularly during inflammation. Elevated ICAM-1 expression is associated with various inflammatory and immune-related conditions, making accurate measurement vital for understanding disease mechanisms and exploring potential therapeutic interventions.
Benefit from Assay Genie's ICAM-1 ELISA Kit, known for its exceptional sensitivity and specificity, ensuring reproducible and dependable results. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly protocols, making it an ideal choice for sensitive and specific detection of ICAM-1 biomarker levels in research studies. Trust Assay Genie's ICAM-1 ELISA Kit for accurate quantification and insightful analysis in your scientific investigations.
Product Name:
Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit
SKU:
AEES00145
Target:
Human ICAM-1/CD54 (intercellular adhesion molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ICAM-1/CD54. You can calculate the concentration of Human ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-104
93-106
99-110
Average (%)
98
99
102
1:4
Range (%)
91-99
95-104
86-97
Average (%)
94
98
92
1:8
Range (%)
83-99
87-102
98-106
Average (%)
91
94
102
1:16
Range (%)
92-104
95-109
87-93
Average (%)
96
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
94
EDTA plasma (n=5)
88-98
95
Cell culture media (n=5)
86-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.42
8.44
0.84
2.29
8.45
Standard deviation
0.06
0.12
0.34
0.05
0.1
0.34
C V (%)
6
4.96
4.03
5.95
4.37
4.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Human ICAM-1/CD54 and analogues was observed.
