The Human IL-10 (Interleukin-10) ELISA Kit is meticulously developed to facilitate the quantitative measurement of IL-10 levels in various biological samples. Interleukin-10, a crucial anti-inflammatory cytokine, plays a vital role in modulating the immune response by suppressing inflammation and promoting immune tolerance. IL-10 is involved in regulating various immune responses, inhibiting pro-inflammatory cytokines, and maintaining immune homeostasis. Accurate quantification of IL-10 levels is essential for understanding its immunomodulatory functions and its implications in inflammation-related disorders. Our ELISA kit offers superior sensitivity and specificity, ensuring accurate and reproducible results crucial for investigating the immunoregulatory roles of IL-10 in different biological contexts. Manufactured under strict quality control measures, this kit delivers robust performance and user-friendly procedures, making it an ideal choice for cutting-edge research applications. Trust Assay Genie's IL-10 ELISA Kit for precise and dependable quantification of this key immunomodulatory biomarker in your studies.
Product Name:
Human IL-10 (Interleukin10) ELISA Kit
SKU:
AEES00162
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
