The Human IL-1 Beta ELISA Kit is a specialized assay designed for the precise quantification of interleukin-1 beta levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Interleukin-1 beta is a key pro-inflammatory cytokine that plays a crucial role in the immune response, inflammation, and various disease processes. Dysregulation of IL-1 beta has been implicated in conditions such as autoimmune diseases, inflammatory disorders, and certain types of cancer. As such, measuring IL-1 beta levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapies.With its advanced technology and reliable performance, the Human IL-1 Beta ELISA Kit is an essential tool for researchers studying inflammatory pathways, immune responses, and disease mechanisms. This kit offers a convenient and efficient way to investigate IL-1 beta levels in human samples, making it a valuable addition to any laboratory's research arsenal.
Product Name:
Human IL-1 beta ELISA Kit
SKU:
HUES01423
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
