The Human IL-2 (Interleukin-2) CLIA Kit is a powerful tool for the quantitative measurement of IL-2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL-2 is a key cytokine that plays a critical role in the regulation of the immune response, specifically in the growth and differentiation of T cells. Dysregulation of IL-2 has been linked to various immune-related disorders, including autoimmune diseases and inflammatory conditions. As such, measuring IL-2 levels can provide valuable insights into the immune system's functioning and help in the development of new therapeutic strategies.Whether you are studying immunology, oncology, or other areas of research, the Human IL-2 CLIA Kit from Assay Genie is an indispensable tool for investigating the role of IL-2 in health and disease. Trust in the accuracy and reliability of this kit to advance your research efforts effectively.
Product Name:
Human IL-2 CLIA Kit
SKU:
HUES00095
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
