The Human IL-3 ELISA Kit is a powerful tool for measuring interleukin-3 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing precise and consistent results for various research applications.Interleukin-3 is a key cytokine that plays a vital role in the regulation of immune responses, hematopoiesis, and inflammation. Abnormal levels of IL-3 have been associated with various diseases, including cancer, autoimmune disorders, and allergic reactions, highlighting its importance as a biomarker for disease diagnosis and treatment monitoring. With its reliable performance and broad utility, the Human IL-3 ELISA Kit is an indispensable tool for researchers exploring the complex role of interleukin-3 in health and disease. Get accurate and insightful data to advance your research with this cutting-edge kit from Assay Genie.
Product Name:
Human IL-3 ELISA Kit
SKU:
HUES01386
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
