The Human IL32 ELISA Kit is specifically designed for the precise and reliable detection of Interleukin-32 (IL-32) levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an excellent tool for various research applications.IL-32 is a key pro-inflammatory cytokine that regulates immune responses and plays a crucial role in inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease, and psoriasis. By measuring IL-32 levels, researchers can gain valuable insights into the immune response and potential therapeutic targets for these conditions.Overall, the Human IL32 ELISA Kit offers researchers a valuable tool for studying the role of IL-32 in inflammation and immune-related disorders, providing a deeper understanding of disease mechanisms and potential treatment strategies.
Product Name:
Human IL-32 ELISA Kit
SKU:
HUES01473
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
