The Human IL-9 ELISA Kit is a powerful tool for measuring IL-9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed for high sensitivity and specificity, providing researchers with accurate and reliable results for a variety of applications.IL-9 is a cytokine that plays a key role in immune responses, particularly in allergic reactions, asthma, and autoimmune diseases. By accurately measuring IL-9 levels, researchers can gain a better understanding of the immune system and develop targeted treatments for these conditions.Whether studying the role of IL-9 in disease pathology or evaluating the effectiveness of potential therapies, the Human IL-9 ELISA Kit is a valuable tool for researchers looking to advance their knowledge in immunology and inflammation.
Product Name:
Human IL-9 ELISA Kit
SKU:
HUES01447
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
