The Human KNG1 (Kininogen-1) CLIA Kit is specifically designed for the quantitative detection of Kininogen-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity for accurate and reproducible results, making it an ideal choice for various research applications.Kininogen-1 is a vital protein involved in the regulation of blood coagulation and inflammation, playing a crucial role in various physiological processes. Dysregulation of Kininogen-1 levels has been implicated in various diseases, including cardiovascular diseases, inflammatory disorders, and cancer.
Thus, the Human KNG1 CLIA Kit serves as a valuable tool for studying the role of Kininogen-1 in these conditions and exploring potential therapeutic interventions.Overall, the Human KNG1 CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for quantifying Kininogen-1 levels, enabling a deeper understanding of its physiological functions and pathological implications.
Product Name:
Human KNG1 (Kininogen 1) CLIA Kit
SKU:
HUES01182
Target:
Human KNG1 (Kininogen 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human KNG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KNG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KNG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human KNG1. You can calculate the concentration of Human KNG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
94-108
93-108
Average (%)
95
100
101
1:4
Range (%)
93-108
94-110
91-101
Average (%)
100
101
96
1:8
Range (%)
90-107
91-109
93-108
Average (%)
98
99
100
1:16
Range (%)
88-99
86-99
95-109
Average (%)
94
91
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
102-115
107
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.95
47.2
149.88
21.51
43.24
153.86
Standard deviation
2.52
5.49
9.17
2.27
3.12
12.59
C V (%)
12.03
11.63
6.12
10.55
7.22
8.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human KNG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KNG1 in samples. No significant cross-reactivity or interference between Human KNG1 and analogues was observed.
