The Human OPG (Osteoprotegerin) ELISA Kit is specially designed to measure OPG levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an excellent tool for various research applications.OPG is a key protein involved in regulating bone metabolism and preventing excessive bone resorption.
Its role in bone-related diseases such as osteoporosis and bone metastasis makes it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Get reliable insights into OPG levels with the Human OPG ELISA Kit, and advance your research in bone health and related fields.
Product Name:
Human OPG (Osteoprotegerin) ELISA Kit
SKU:
HUES02388
Target:
Human OPG (Osteoprotegerin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OPG. You can calculate the concentration of Human OPG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
87-99
88-99
Average (%)
94
92
94
1:4
Range (%)
97-115
82-94
96-114
Average (%)
105
89
104
1:8
Range (%)
101-116
85-101
94-109
Average (%)
106
92
100
1:16
Range (%)
96-110
82-91
94-109
Average (%)
104
87
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
101
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
5.0
0.5
1.1
4.8
Standard deviation
0.03
0.06
0.18
0.03
0.05
0.17
C V (%)
6
5.0
3.6
6.0
4.55
3.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OPG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OPG in samples. No significant cross-reactivity or interference between Human OPG and analogues was observed.
