The Human OPG (Osteoprotegerin) ELISA Kit is specially designed to measure OPG levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an excellent tool for various research applications.OPG is a key protein involved in regulating bone metabolism and preventing excessive bone resorption. Its role in bone-related diseases such as osteoporosis and bone metastasis makes it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Get reliable insights into OPG levels with the Human OPG ELISA Kit, and advance your research in bone health and related fields.
Product Name:
Human OPG (Osteoprotegerin) ELISA Kit
SKU:
HUES02388
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
