Human Parathyroid Hormone Related Protein (PTHrP) ELISA Kit
The Human Parathyroid Hormone Related Protein (PTHrP) ELISA Kit is a reliable tool for the precise measurement of PTHrP levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.PTHrP is a key regulatory protein that plays a critical role in calcium homeostasis and bone metabolism. Dysregulation of PTHrP levels has been linked to various disorders, such as hypercalcemia, bone metastasis, and certain cancers.
Consequently, monitoring PTHrP levels can provide valuable insights into these conditions and aid in the development of therapeutic interventions.Overall, the Human PTHrP ELISA Kit is a valuable tool for researchers and clinicians investigating the role of PTHrP in health and disease, offering precise and reliable measurements to advance our understanding of this important protein.
Product Name:
Human Parathyroid Hormone Related Protein (PTHrP) ELISA Kit
Product Code:
HUDL02452
Size:
96 Assays
Target:
PTHrP
Synonyms:
PTHLH, HHM, PTHR, PLP, Osteostatin, Parathyroid Hormone Like Hormone
Detection Method:
Sandwich
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
91-99
95
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
