The Human POSTN/OSF-2 (Periostin) ELISA Kit is expertly crafted for the precise quantification of Periostin (POSTN/OSF-2) levels in various biological samples. Periostin is a multifunctional matrix protein crucial in tissue repair, cell adhesion, and tissue remodeling processes, making it a key player in extracellular matrix regulation and various pathophysiological conditions. Accurate measurement of POSTN/OSF-2 levels is essential for understanding its role in tissue homeostasis, inflammation, and disease progression, providing valuable insights for targeted intervention strategies. With exceptional sensitivity and specificity, our POSTN/OSF-2 ELISA Kit ensures accurate and reproducible results, meeting the highest standards of quality control. Designed for robust performance and user-friendly operation, this kit is ideal for cutting-edge research applications. Trust Assay Genie's POSTN/OSF-2 ELISA Kit for dependable quantification of this critical biomarker in your studies.
Product Name:
Human POSTN/OSF-2 (Periostin) ELISA Kit
SKU:
AEES00164
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.