The Human PSPN (Persephin) CLIA Kit is specifically designed to accurately measure levels of persephin in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.Persephin is a key protein involved in the development and maintenance of the nervous system, particularly in the survival and differentiation of dopaminergic neurons. Its role in neurodegenerative disorders, such as Parkinson's disease, makes it a valuable biomarker for studying these conditions and potentially developing new treatments.
Overall, the Human PSPN (Persephin) CLIA Kit offers researchers a powerful tool for investigating the functions and roles of persephin in various physiological and pathological processes, helping to advance our understanding of neurodegenerative diseases and potentially leading to new therapeutic approaches.
Product Name:
Human PSPN (Persephin) CLIA Kit
SKU:
HUES00932
Target:
Human PSPN (Persephin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PSPN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PSPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PSPN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PSPN. You can calculate the concentration of Human PSPN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
86-100
99-114
Average (%)
98
92
107
1:4
Range (%)
88-102
87-98
94-107
Average (%)
95
93
101
1:8
Range (%)
86-101
86-100
99-113
Average (%)
93
92
105
1:16
Range (%)
92-106
89-101
88-101
Average (%)
97
94
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.62
63.63
192.73
18.92
59.51
211.79
Standard deviation
1.83
5.7
21.89
2.37
5.18
19.74
C V (%)
8.87
8.96
11.36
12.53
8.7
9.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PSPN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PSPN in samples. No significant cross-reactivity or interference between Human PSPN and analogues was observed.
