Human Solute Carrier Family 30, Member 10 (SLC30A10) ELISA Kit
The Human SLC30A10 ELISA Kit is specifically designed for the precise measurement of Solute Carrier Family 30 Member 10 (SLC30A10) levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SLC30A10 is a critical protein involved in regulating zinc transport and homeostasis within the body. Dysregulation of SLC30A10 has been linked to various health conditions including metabolic disorders, neurodegenerative diseases, and other zinc-related disorders.
Therefore, the measurement of SLC30A10 levels can serve as a valuable biomarker for studying these conditions and developing potential treatment strategies.Overall, the Human SLC30A10 ELISA Kit provides researchers with a reliable tool for investigating the role of SLC30A10 in health and disease, ultimately advancing our understanding of zinc metabolism and associated disorders.
Product Name:
Human Solute Carrier Family 30, Member 10 (SLC30A10) ELISA Kit
Product Code:
HUDL02701
Size:
96 Assays
Target:
SLC30A10
Synonyms:
ZnT-10, ZRC1, ZNT8, Zinc Transporter 8
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
