The Human TG (Thyroglobulin) ELISA Kit is designed for the quantitative detection of Thyroglobulin levels in various biological samples. Thyroglobulin is a key protein involved in thyroid hormone synthesis within the thyroid gland and is essential for normal thyroid function. It serves as the precursor for the production of thyroxine (T4) and triiodothyronine (T3), which play crucial roles in regulating metabolism, growth, and development in the body. Accurate measurement of Thyroglobulin levels is important in assessing thyroid health, monitoring thyroid diseases, and evaluating treatment outcomes. This ELISA kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, the kit delivers robust performance and user-friendly protocols, making it suitable for both research and clinical applications. Trust the Human TG ELISA Kit for precise quantification of this vital biomarker in your studies.
Product Name:
Human TG (Thyroglobulin) ELISA Kit
SKU:
AEES00113
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
