The ICAM-1/CD54 Polyclonal Antibody (CAB13620) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ICAM-1/CD54 Polyclonal Antibody
SKU:
CAB13620
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BB2, CD54, P3.58, ICAM-1/CD54
Positive Sample:
Raji
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
58kDa
Observed MW:
92kDa
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
Purification Method
Affinity purification
Gene ID
3383
RRID
AB_2760481
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using ICAM-1/CD54 Rabbit pAb (CAB13620) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
![Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214503/569409/anti-mouse-icam-1-cd54-be29g1-in-vivo-antibody-low-endotoxin__35728.1673496775.jpg?c=2 "Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Low Endotoxin")
