Mouse Acetyl Coenzyme A Carboxylase Alpha (ACACa) ELISA Kit
Based on the information in the provided URL for the Mouse Acetyl-Coenzyme A Carboxylase Alpha (ACACA) ELISA Kit from Assay Genie, we can create the following product description: The Mouse Acetyl-Coenzyme A Carboxylase Alpha (ACACA) ELISA Kit from Assay Genie is specifically designed for the accurate and precise measurement of ACACA levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications. ACACA plays a crucial role in fatty acid synthesis and energy metabolism, making it a key enzyme in processes such as lipid metabolism and insulin signaling.
Dysregulation of ACACA has been associated with metabolic disorders, obesity, and various other diseases, highlighting its importance as a potential therapeutic target and biomarker for monitoring disease progression. With the Assay Genie Mouse ACACA ELISA Kit, researchers can confidently study the role of ACACA in various physiological and pathological conditions, paving the way for new insights into metabolic diseases and potential therapeutic interventions.
Product Name:
Mouse Acetyl Coenzyme A Carboxylase Alpha (ACACa) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
