The Mouse AMH (Anti-Mullerian Hormone) CLIA Kit is specifically designed for the accurate quantification of anti-Mullerian hormone levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Anti-Mullerian hormone is a key regulator of reproductive development, playing a crucial role in the formation of male and female sex organs.
Its levels are known to fluctuate in response to various physiological and pathological conditions, making it a valuable biomarker for studying reproductive disorders and potential treatment options.With the Mouse AMH CLIA Kit, researchers can confidently measure anti-Mullerian hormone levels in mouse samples with ease and reliability, providing valuable insights into reproductive biology and related research areas.
Product Name:
Mouse AMH (Anti-Mullerian Hormone) CLIA Kit
SKU:
MOES00079
Target:
Mouse AMH (Anti-Mullerian Hormone)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AMH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AMH. You can calculate the concentration of Mouse AMH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
98-114
85-99
Average (%)
108
105
91
1:4
Range (%)
99-114
96-109
101-114
Average (%)
105
103
108
1:8
Range (%)
99-114
89-104
100-116
Average (%)
107
96
107
1:16
Range (%)
97-109
96-108
98-113
Average (%)
103
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
99-115
105
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.52
527.86
1764.76
208.11
567.24
1626.62
Standard deviation
16.46
47.14
156.18
18.0
52.47
122.16
C V (%)
8.05
8.93
8.85
8.65
9.25
7.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AMH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AMH in samples. No significant cross-reactivity or interference between Mouse AMH and analogues was observed.
