The Mouse APOC3 (Apolipoprotein C3) ELISA Kit is a reliable tool for the quantitative detection of APOC3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.APOC3 is a key protein involved in lipid metabolism and is known to play a role in regulating triglyceride levels. Dysregulation of APOC3 has been linked to conditions such as hypertriglyceridemia and cardiovascular diseases, making it a valuable biomarker for studying these disorders and exploring potential therapeutic interventions.
With easy-to-follow protocols and efficient assay performance, the Mouse APOC3 ELISA Kit is an essential tool for researchers interested in studying lipid metabolism and its implications in various health conditions.
Product Name:
Mouse ApoC3 (Apolipoprotein C3) ELISA Kit
SKU:
MOES01741
Target:
Mouse ApoC3 (Apolipoprotein C3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoC3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoC3. You can calculate the concentration of Mouse ApoC3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
95-110
95-106
Average (%)
94
102
101
1:4
Range (%)
98-115
87-99
96-111
Average (%)
106
94
104
1:8
Range (%)
96-110
84-100
92-107
Average (%)
101
91
99
1:16
Range (%)
101-118
87-100
94-110
Average (%)
108
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.8
7.8
0.9
2.7
7.6
Standard deviation
0.08
0.13
0.41
0.05
0.14
0.39
C V (%)
8
4.64
5.26
5.56
5.19
5.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ApoC3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoC3 in samples. No significant cross-reactivity or interference between Mouse ApoC3 and analogues was observed.
