The Mouse C3 (Complement 3) ELISA Kit is specifically designed for the precise measurement of complement 3 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it perfect for various research purposes.Complement 3 is a vital component of the complement system, which plays a crucial role in the body's immune response. It is involved in various processes such as inflammation, opsonization, and cell lysis. Dysregulation of complement 3 has been linked to several diseases, including autoimmune disorders, infections, and inflammatory conditions.Overall, the Mouse C3 ELISA Kit is a valuable tool for researchers studying the role of complement 3 in health and disease, offering accurate and reliable data for further investigation and potential therapeutic development.
Product Name:
Mouse C3 (Complement 3) ELISA Kit
SKU:
MOES00893
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 3 vials, 20 mL | 96T*5: 15 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
92-104
99-112
95-108
Average (%)
99
106
102
1:4
Range (%)
88-102
87-97
87-102
Average (%)
95
92
93
1:8
Range (%)
89-104
88-98
87-102
Average (%)
96
93
93
1:16
Range (%)
85-99
86-98
86-100
Average (%)
92
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
91-107
99
Cell Culture Media (n=8)
91-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.12
30.83
70.91
8.99
33.18
68.73
Standard deviation
0.53
1.25
3.3
0.73
2.5
4.63
C V (%)
5.86
4.04
4.66
8.07
7.53
6.73
Uniport ID:
P01027
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse C3 in samples. No significant cross-reactivity or interference between Mouse C3 and analogues was observed
Storage:
2-8℃
Research Area:
Cancer,Immunology,Signal Transduction
Leavy, A., et al.
Investigating Pharmacological Inhibition of the NLRP3 Inflammasome Acutely and Chronically Post Hypoxia in a Moderate Model of Hypoxic-Ischaemic Encephalopathy
