The Mouse C3a Complement Component 3a ELISA Kit is specifically designed to accurately measure levels of C3a complement component 3a in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.C3a complement component 3a is a key player in the complement system, a crucial component of the immune response that helps to clear pathogens and promote inflammation. Dysregulation of the complement system has been linked to various diseases, including autoimmune disorders, infections, and inflammatory conditions. Therefore, the measurement of C3a levels is important for understanding the role of this protein in health and disease, as well as for the development of potential therapeutic interventions.Overall, the Mouse C3a Complement Component 3a ELISA Kit from Assay Genie provides researchers with a valuable tool for investigating the function and regulation of C3a in mouse models, offering reliable and accurate results for a wide range of experimental settings.
Product Name:
Mouse C3a (Complement Component 3a) ELISA Kit
SKU:
MOES00900
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
