The Mouse CFH (Complement Factor H) ELISA Kit is a specialized assay designed for the precise and quantitative detection of Complement Factor H levels in mouse biological samples. Complement Factor H is a key regulator of the innate immune response, playing a critical role in the modulation of the complement system, host defense, and inflammation. Our ELISA kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results for researchers studying the intricate mechanisms of the immune system and inflammatory responses. With stringent quality control measures in place, this kit delivers robust performance, making it an ideal choice for investigations focused on the functions and implications of Complement Factor H in various physiological and pathological conditions.
Product Name:
Mouse CFH (Complement Factor H) ELISA Kit
SKU:
AEES00216
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
