The Mouse Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit is a reliable and sensitive tool for detecting levels of EAAT2 in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring consistent and reproducible results for various research applications.EAAT2 is an important transporter protein that regulates the levels of excitatory neurotransmitters in the brain, such as glutamate. Dysregulation of EAAT2 has been implicated in various neurological disorders, including epilepsy, Alzheimer's disease, and amyotrophic lateral sclerosis (ALS).
Therefore, accurate measurement of EAAT2 levels is crucial for studying these conditions and developing potential therapeutic interventions.This ELISA kit provides researchers with a valuable tool for quantifying EAAT2 levels in mouse samples, ultimately advancing our understanding of neurological disorders and facilitating the development of novel treatment strategies.
SLC1A2, EAAT2, GLT-1, Solute Carrier Family 1 Member 2, Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2
Detection Method:
Sandwich
Range:
0.156-10 ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
