The Mouse GOT2 (Aspartate Aminotransferase) ELISA Kit is designed for the precise measurement of aspartate aminotransferase levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for various research applications. Aspartate aminotransferase is an important enzyme involved in amino acid metabolism, playing a key role in the conversion of aspartate to oxaloacetate.
Abnormal levels of this enzyme can indicate liver damage, heart disease, or muscle injury, making it a valuable biomarker for studying these conditions and developing potential treatments. Overall, the Mouse GOT2 ELISA Kit is a reliable tool for researchers looking to investigate the role of aspartate aminotransferase in various biological processes and disease states in mouse models.
Product Name:
Mouse GOT2/Aspartate aminotransferase ELISA Kit
SKU:
MOFI00468
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-105%
87-103%
85-104%
EDTA Plasma (n = 5)
83-96%
84-101%
84-101%
Heparin Plasma (n = 5)
82-95%
81-90%
82-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-104
96
EDTA Plasma (n = 5)
88-99
95
Heparin Plasma (n = 5)
89-101
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
