The Mouse Haptoglobin (HPT) ELISA Kit is a powerful tool for the precise quantification of haptoglobin levels in mouse serum, plasma, and cell culture supernatants. This kit is designed with exceptional sensitivity and selectivity, ensuring accurate and consistent results for a variety of research investigations.Haptoglobin is a key protein involved in the immune response, acting as a scavenger for free hemoglobin and playing a vital role in inflammation and oxidative stress. Dysregulation of haptoglobin levels has been linked to various diseases, including inflammatory disorders, cardiovascular diseases, and cancer, making it a valuable biomarker for studying these pathologies and exploring potential therapeutic interventions.
With its high performance and reliability, the Mouse Haptoglobin (HPT) ELISA Kit is an indispensable tool for researchers seeking to deepen their understanding of haptoglobin biology and its implications in disease processes.
Product Name:
Mouse Haptoglobin (Hpt) ELISA Kit
Product Code:
MODL00629
Size:
96 Assays
Target:
Hpt
Synonyms:
HP, Hp2-Alpha, Alpha-2-Macroglobulin, Zonulin
Detection Method:
Sandwich
Range:
31.25-2,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
