The Mouse IL-1 Beta ELISA Kit is specifically designed for the precise measurement of interleukin-1 beta levels in mouse serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and consistent results, making it perfect for a variety of research applications.Interleukin-1 beta is a key cytokine that plays a crucial role in inflammation and immune response regulation. It is involved in various physiological and pathological processes, including infection, autoimmune diseases, and inflammatory conditions. This ELISA kit allows researchers to study the role of interleukin-1 beta in disease progression and develop potential therapeutic strategies.
Product Name:
Mouse IL-1 beta ELISA Kit
SKU:
MOES00657
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
