The Mouse IL-16 ELISA Kit is a powerful tool for measuring levels of interleukin-16 (IL-16) in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering precise and consistent results for a variety of research purposes.IL-16 is a key cytokine involved in regulating immune responses and inflammatory processes. It plays a critical role in modulating T cell activation and migration, making it a valuable biomarker in studying autoimmune diseases, allergies, and inflammatory conditions. The Mouse IL-16 ELISA Kit provides researchers with a reliable method for analyzing IL-16 levels in experimental samples, aiding in the understanding of immune system dynamics and disease mechanisms.
Product Name:
Mouse IL-16 ELISA Kit
SKU:
MOES01224
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
