The Mouse IL-3 ELISA Kit is a powerful tool for detecting and quantifying Interleukin-3 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.Interleukin-3 is a vital cytokine that regulates the growth and differentiation of a variety of immune cells, including mast cells, eosinophils, and basophils. It is also involved in inflammation and allergic responses, making it a key target for studying immune-related diseases and developing new therapeutic strategies. Whether investigating immune system function, studying inflammatory diseases, or exploring potential treatments, the Mouse IL-3 ELISA Kit is an essential tool for researchers seeking to better understand the role of Interleukin-3 in mouse models. Order yours today and take your research to the next level.
Product Name:
Mouse IL-3 ELISA Kit
SKU:
MOES01216
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
