The Mouse Interleukin-7 (IL7) ELISA Kit is specifically designed for the precise detection of interleukin-7 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it the perfect tool for a variety of research applications.Interleukin-7 is a key cytokine that plays a crucial role in immune function, particularly in the development and maintenance of T cells.
Dysregulation of IL7 has been implicated in a variety of immune-related disorders, making it an important marker for studying these conditions and potentially identifying new therapeutic targets.With the Mouse Interleukin-7 (IL7) ELISA Kit, researchers can confidently measure IL7 levels in mouse samples with precision and reliability, advancing their understanding of immune response and potentially leading to new insights in the field of immunology.
Product Name:
Mouse Interleukin 7 (IL-7) ELISA Kit
Product Code:
MODL00716
Size:
96 Assays
Target:
IL7
Synonyms:
Pre-B-cell Growth Factor, Lymphopoietin-1
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
Fei Li et al.
Severe persistent mycobacteria antigen stimulation causes lymphopenia through impairing hematopoiesis
Frontiers in Cellular and Infection Microbiology 2023
