The Mouse PKC Alpha (PRKCA) ELISA Kit is expertly designed for the precise measurement of protein kinase C alpha (PKC alpha) levels in mouse samples, including serum, plasma, and cell culture supernatants. This ELISA kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for various research applications. PKC alpha is a critical enzyme involved in signal transduction, cell growth, and differentiation. Dysregulation of PKC alpha has been linked to numerous diseases, including cancer, diabetes, and cardiovascular disorders, highlighting its importance as a biomarker for studying these conditions and potentially developing targeted treatments.
This Mouse PKC Alpha (PRKCA) ELISA Kit is an indispensable tool for researchers looking to explore the role of PKC alpha in health and disease, providing reliable data to advance scientific understanding and therapeutic interventions.
Product Name:
Mouse PKC alpha/PRKCA ELISA Kit
SKU:
MOFI00499
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-105%
82-99%
82-97%
EDTA Plasma (n = 5)
85-104%
82-97%
82-97%
Heparin Plasma (n = 5)
95-105%
82-89%
84-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-105
96
EDTA Plasma (n = 5)
94-104
99
Heparin Plasma (n = 5)
89-95
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positi
